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Image Search Results
Journal: Arthritis Research & Therapy
Article Title: Upregulated endonuclease Regnase-1 suppresses osteoarthritis by forming a negative feedback loop of catabolic signaling in chondrocytes
doi: 10.1186/s13075-021-02485-z
Figure Lengend Snippet: SAA3 is a target of Regnase-1 in chondrocyte. a , b Number of genes modulated by Regnase-1 ( a ) and list of the top 13 genes that were most markedly regulated by Ad-shRegnase-1 ( b ). c Luciferase assay ( n = 4) in chondrocytes transfected with a reporter plasmid containing the SAA3 3′-UTR sequence. Chondrocytes were co-transfected with empty vector (EV), WT Regnase-1 (WT), or D141N Regnase-1 (D141N). d qRT-PCR analysis ( n = 6) of SAA family members in chondrocytes infected with Ad-Regnase-1 or Ad-shRegnase-1 for 36 h. e Quantitation of secreted SAAs by ELISA in chondrocytes treated with IL-1β (0.1 ng/ml, 36 h) or infected with the indicated MOI of Ad-C or Ad-SAA3 (36 h) ( n = 4). Means ± s.e.m. and one-way ANOVA with Bonferroni’s post hoc test. * p < 0.05, ** p < 0.005, *** p < 0.0005
Article Snippet: The SAA levels were analyzed using a
Techniques: Luciferase, Transfection, Plasmid Preparation, Sequencing, Quantitative RT-PCR, Infection, Quantitation Assay, Enzyme-linked Immunosorbent Assay
Journal: International Journal of Molecular Sciences
Article Title: Effect of Hyaluronic Acid and Mesenchymal Stem Cells Secretome Combination in Promoting Alveolar Regeneration
doi: 10.3390/ijms24043642
Figure Lengend Snippet: Quantitative expression of SPC ( A ), SP-B ( B ) and SPA ( C ) and SPD ( D ) performed by Elisa kit test, after the incorporation of secretome in combination with HA in SAGM.
Article Snippet: Human pulmonary surfactant associated protein (SP) SP-A, SP-B, SPC and
Techniques: Expressing, Enzyme-linked Immunosorbent Assay